Speaker
Description
The migration of molecules and cellular organelles is essential for cellular functions. However, analysing such dynamics is challenging due to the high spatial and temporal resolution required and the accurate analysis of the diffusional tracks. Here, we investigate the migration modes of peroxisome organelles in the cytosol of living cells. Peroxisomes predominantly migrate randomly, but occasionally, they bind to the cell's microtubular network and perform directed migration. So far, an accurate analysis of switching between these migration modes is missing. At a high acquisition rate, we collect temporal diffusion tracks of thousands of individual peroxisomes in the HEK-293 cell line using spinning disc fluorescence microscopy. We use a Hidden Markov Model (HMM) to i) automatically identify directed migration in the tracks and ii) quantify the migration properties to compare different experimental conditions. Comparing different cellular conditions, we show that the knockout of the peroxisomal membrane protein PEX14 leads to decreased directed movement due to a lowered binding probability to the microtubule. Structural changes in the microtubular network explain Nocodazole-treatment's perceived eradication of directed movement.
| Authors | Carl-Magnus Svensson*, Katharina Reglinski, Wolfgang Schliebs, Ralf Erdmann, Christian Eggeling, Marc Thilo Figge |
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| Keywords | Track analysis, Hidden Markov Model, Subcellular migration |
