Speaker
Alán Fernando Muñoz González
(Broad Institute of Harvard and MIT)
Description
With the Cell Painting assay we quantify cell morphology using six dyes to stain eight cellular components: Nucleus, mitochondria, endoplasmic reticulum, nucleoli, cytoplasmic RNA, actin, golgi aparatus, and plasma membrane. After high-throughput fluorescence microscopy, image analysis algorithms then extract thousands of morphological features from each single cell’s image. By comparing of these “profiles” we can can uncover new relationships among genetic and chemical perturbations.
The JUMP-CP Consortium (Joint Undertaking for Morphological Profiling-Cell Painting) released the first public high-throughput dataset with over 140,000 genetic and chemical perturbations.
| Authors | Alán F. Muñoz*, The Broad Institute's Imaging Platform, Shantanu Singh, Anne E. Carpenter |
|---|---|
| Keywords | high-throughput, microscopy, cellpainting, software |
