Speaker
Description
The mammalian cerebral cortex arises from neocortical and hippocampal primordia (Ncp and Hcp respectively), which initially display cellular composition similarities and several molecular markers. Their trajectories diverge significantly during development, resulting in functionally distinct structures. The molecular mechanisms orchestrating the progressive regionalization of the Ncp and Hcp remain poorly understood. Using single-cell multiomic analysis, we demonstrate significant differences in the Ncp and Hcp as early as E12.5, regarding chromatin accessibility and transcriptional profiles. In particular, we identified differentially accessible enhancer regions in apical progenitors from Ncp and Hcp harboring both known and previously undescribed binding motifs for key transcriptional regulators. Ongoing work is focused on functionally validating some of these enhancer regions that modulate gene expression and may drive the gradual diversification of the Ncp and Hcp. Our findings provide critical mechanistic insights into early chromatin regulation underlying region-specific transcriptional programs that result in the functional divergence of the neocortex and hippocampus.
| Author(s) | Mahima Bose1,*, Faye Chong2,3,*, Varun Suresh1, Samhita Ramanan Radhakrishnan1, Tamar Sapir4, Orly Reiner4, Boyan Bonev2,3,#, and #Shubha Tole1,# |
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| Affiliation(s) | "1 Department of Biological Sciences, Tata Institute of Fundamental Research, Mumbai, India; 2 Biomedical Center Munich (BMC), Physiological Genomics, LMU Munich, Planegg, Germany; 3 Helmholtz Pioneer Campus, Helmholtz Center Munich, Neuherberg, Germany; 4 Department of Molecular Neuroscience, Weizmann Institute of Science, Rehovot, Israel. *equally contributing co-authors # co-corresponding authors" |
